Investigations on ploidy levels of haploid and diploid callus and cell suspension cultures of Datura innoxia Mill.

نویسندگان

  • R Kibler
  • J Blaschke
  • E Forche
  • K H Neumann
چکیده

Using haploid and diploid Datura innoxia Mill, callus cultures and cell suspensions it was shown that meristematic material usually attains the lowest possible C-value of a given ploidy level. Parenchyma material of callus cultures, however, indicates a broad scattering of C-values up to 16 C, which is paralleled by C-value distribution of the free cell fraction in actively dividing cell suspensions. In these suspensions, cell division activity seems to be restricted to small meristem-like clusters.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Herbicide Resistance in Datura innoxia: Cross-Resistance of Sulfonylurea-Resistant Cell Lines to Imidazolinones.

Cells resistant to the sulfonylurea herbicides chlorsulfuron and sulfometuron methyl were isolated from a predominantly haploid cell suspension culture of Datura innoxia P. Mill. Exponentially growing cell colonies (aggregates of about 40 cells) were mutagenized with ethyl methane sulfonate, subcultured for 10 days to allow growth recovery and plated on a medium containing either chlorsulfuron ...

متن کامل

Investigation on primary and secondary metabolism of Datura innoxia Mill. tissue cultures

in journal: 1, László I., Szoke É. and Tyihák E. (1998) Effect of Magnesium on the Methylation of Alkaloids in Datura innoxia Mill. Tissue Cultures. Magnesium Research, 11, 236-237.

متن کامل

Expression of 5-Methyltryptophan Resistance in Plants Regenerated from Resistant Cell Lines of Datura innoxia.

Seventy-nine 5-methyltryptophan-resistant cell lines have been selected from haploid Datura innoxia Mill. cell cultures by plating suspensions in agar medium containing a growth inhibitory concentration of 5-methyltryptophan. Mutagen treatment increased the frequency of resistance. The eleven variants tested posses an altered anthranilate synthase less sensitive to feedback inhibition by trypto...

متن کامل

Selection, Isolation, and Characterization of Cadmium-Resistant Datura innoxia Suspension Cultures.

Datura innoxia cells from suspension cultures were selected for their ability to grow and divide rapidly in normally lethal concentrations of cadmium. Cells resistant to 12.5, 25, 50, 100, 160, 200, and 250 micromolar cadmium chloride were isolated and utilized to initiate cell suspension cultures resistant to this toxic metal ion. Variant cell lines retained their ability to grow in cadmium af...

متن کامل

In vitro organogenesis and alkaloid accumulation in Datura innoxia.

The kinetics of tropane alkaloids accumulation in different organs such as roots, leaves, stems, flowers and seeds of Datura innoxia was investigated by GC-MS. Twenty-six tropane alkaloids were detected. The ester derivatives of tropine (3alpha-tigloyloxytropine and 3-tigloyloxy-6-hydroxytropine) are the major compounds. Undifferentiated callus were established from the stem explants of Datura ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of cell science

دوره 44  شماره 

صفحات  -

تاریخ انتشار 1980